LOCUS       40924_29511        5347 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pLYS1B DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pLYS1B
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5347)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5347)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..956
                     /pseudo
                     /gene="lys1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..956
                     /pseudo
                     /codon_start=1
                     /gene="lys1"
                     /label=lys1
                     /note="NotI site is created at the middle of this fragment 
                     (613-620)."
                     /translation="VLCSTCAVSRSNIFGIAKRCHSSRTWHEECPTFGDQSF*Y*QDMR
                     YRRSW*NISSCWWIG*GLPWK**ID*QKILKELVC*SLQVCRPNSRKCALETLLVRNTR
                     SYVSFW*LGPLSPNW*CRM*RPPTIKLKFVVSELS*VKLIPIFLVILMSVKILPLFDVI
                     RMKNPR*LHTLYLKV*IKMTLIALLSRKILSSMV*RSTGSLYMIYGNILRLNFLVMLFL
                     L*LFPFIKCLSILMER"
     gene            242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
     promoter        962..1369
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1376..1423
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1442..1837
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      1934..2181
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2207..2223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2436..2891
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3173..3277
                     /label=AmpR promoter
     CDS             3278..4135
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4309..4897
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5185..5206
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5221..5251
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5259..5275
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5283..5299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtactttgt agtacctgcg cggtctcaag atcaaacatt tttggaatcg caaaaagatg
      301 tcattccagc cggacgtggc atgaagaatg tccaactttt ggtgatcaat cgttttgata
      361 ctaacaagat atgcggtatc ggagaagttg gtgaaatata tcttcgtgct ggtggattgg
      421 ctgagggcta ccttggaaat gatgaattga ctagcaaaaa attcttaaag agttggtttg
      481 ctgatccctc caagtttgta gaccgaactc cagaaaatgc gccctggaaa ccttattggt
      541 tcggaatacg cgatcgtatg tatcgttctg gtgacttggg ccgctatctc ccaactggta
      601 atgtagaatg tagcggccgc cgacgatcaa attaaaattc gtggtttccg aattgagtta
      661 ggtgaaatta atacccatct ttctcgtcat cctaatgtcc gtgaaaatat tacccttgtt
      721 cgacgtgata aggatgaaga acccacgcta gttgcataca ttgtacctca aggtttaaat
      781 aaagatgact ttgatagcgc tactgagtcg gaagatattg tcgtcaatgg tttgaagaag
      841 tacaggaagc ttatacatga tatacgggaa tatcttaaga ctaaacttcc tagttatgct
      901 attccttctg taattgttcc ccttcataaa atgcctctca atcctaatgg aaagatctag
      961 cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
     1021 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
     1081 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga
     1141 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
     1201 tcttgaaaat tttttttttt agtttttttc tctttcgatg acctcccatt gatatttaag
     1261 ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact
     1321 ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagg gcggcgttga
     1381 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     1441 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     1501 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     1561 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     1621 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     1681 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     1741 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     1801 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc
     1861 aggactgaca cgtccgacgc ggcccgacgg gtccgaggcc tcggagatcc gtcccccttt
     1921 tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     1981 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     2041 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     2101 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     2161 cgctcgaagg ctttaatttg caagctgttt aaacgagctc gaattcactg gccgtcgttt
     2221 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2281 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2341 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     2401 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     2461 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2521 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2581 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2641 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2701 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2761 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     2821 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2881 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2941 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3001 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3061 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3121 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3181 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3241 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3301 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3361 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3421 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3481 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3541 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3601 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3661 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3721 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3781 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3841 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3901 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3961 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4021 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4081 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4141 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4201 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4441 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4921 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4981 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5041 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5101 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5161 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5221 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5281 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
     5341 aggatcc
//
